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Addgene

pMMP-puro-Flag-HA-NR6A1
(Plasmid #46833)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46833 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMMP-puro
  • Backbone manufacturer
    NA
  • Backbone size w/o insert (bp) 7511
  • Total vector size (bp) 9077
  • Modifications to backbone
    Insertion of NR6A1 cDNA with N-terminal Flag-HA-epitope and 8-residue linker.
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NR6A1
  • Alt name
    GCNF
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1566
  • Mutation
    None
  • Entrez Gene
    Nr6a1 (a.k.a. 1700113M01Rik, Gcn, Gcnf, NC, NCNF, RTR)
  • Tag / Fusion Protein
    • Flag-HA, plus 8-amino acid residue linker (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Insert-specific primer
  • 3′ sequencing primer Insert-specific primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMP-puro-Flag-HA-NR6A1 was a gift from Phil Sharp (Addgene plasmid # 46833 ; http://n2t.net/addgene:46833 ; RRID:Addgene_46833)
  • For your References section:

    Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts. Gurtan AM, Ravi A, Rahl PB, Bosson AD, JnBaptiste CK, Bhutkar A, Whittaker CA, Young RA, Sharp PA. Genes Dev. 2013 Apr 15;27(8):941-54. doi: 10.1101/gad.215376.113. 10.1101/gad.215376.113 PubMed 23630078