pMMP-puro-Flag-HA-NR6A1
(Plasmid
#46833)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMMP-puro
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Backbone manufacturerNA
- Backbone size w/o insert (bp) 7511
- Total vector size (bp) 9077
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Modifications to backboneInsertion of NR6A1 cDNA with N-terminal Flag-HA-epitope and 8-residue linker.
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNR6A1
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Alt nameGCNF
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SpeciesM. musculus (mouse)
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Insert Size (bp)1566
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MutationNone
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Entrez GeneNr6a1 (a.k.a. 1700113M01Rik, Gcn, Gcnf, NC, NCNF, RTR)
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Tag
/ Fusion Protein
- Flag-HA, plus 8-amino acid residue linker (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (destroyed during cloning)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Insert-specific primer
- 3′ sequencing primer Insert-specific primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMMP-puro-Flag-HA-NR6A1 was a gift from Phil Sharp (Addgene plasmid # 46833 ; http://n2t.net/addgene:46833 ; RRID:Addgene_46833) -
For your References section:
Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts. Gurtan AM, Ravi A, Rahl PB, Bosson AD, JnBaptiste CK, Bhutkar A, Whittaker CA, Young RA, Sharp PA. Genes Dev. 2013 Apr 15;27(8):941-54. doi: 10.1101/gad.215376.113. 10.1101/gad.215376.113 PubMed 23630078
Map uploaded by the depositor.