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Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
UseTagsExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis
Plasmid#156662PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertKIR2DL4 (KIR2DL4 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LILRB1-Fc(DAPA)-AviTag-6xHis
Plasmid#156862PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertLILRB1 (LILRB1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFR-PATagRFP
Plasmid#31950DepositorInsertPATagRFP
UseTagsEGF receptor (EGFR)ExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LILRB2-Fc(DAPA)-AviTag-6xHis
Plasmid#156863PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertLILRB2 (LILRB2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
M3-PAmcherry1
Plasmid#114189Purposestudying clustering of M3 receptors by superresolution microscopy (PALM)DepositorInsertMuscarin acetylcholine receptor 3 (CHRM3 Human)
UseTags3xHA and PAmCherryExpressionMammalianMutationPromoterSV40Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-TGNP-N-10
Plasmid#54279PurposeLocalization: Golgi Complex, Excitation: 487, Emission: 509DepositorInsertTGNP (TGOLN2 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-TFEB-WT-MYC (#647)
Plasmid#99955Purposemammalian expressionDepositorInsertTFEB (TFEB Human)
UseTags6xHis and MycExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA
Plasmid#176270PurposeExpression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cellsDepositorInsertTadA-TadA*(8e)-dCasMINI-V4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
eMagAF-EGFP-PM
Plasmid#162247PurposeBlue-light dependent heterodimerization systemDepositorInserteMagAF-EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 human p27
Plasmid#14049DepositorInsertp27 (CDKN1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-zGrad
Plasmid#119716PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafishDepositorInsertzGrad
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-3xHA
Plasmid#102930PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsHAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1
Plasmid#20668PurposeS. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.DepositorTypeEmpty backboneUseTagsGFP mut3ExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pegRNA2_tevopreQ1
Plasmid#212039PurposeBacterial expression of the pegRNA for genome editing in E. coliDepositorInsertpegRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PTRE-tight-hM3Dq-mCherry
Plasmid#66795PurposeExpresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTagingDepositorInsertpTIGHT promoter-hM3Dq-mCherry
UseAAVTagsExpressionMammalianMutationPromoterpTIGHT TREAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PKM2
Plasmid#64698PurposeExpression of GFP tagged PKM2 in mammalian cellsDepositorInsertPKM2 (PKM Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-WT
Plasmid#133749PurposeWild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimdDepositorInsertWild type EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGolt-mCherry / pGolt3-mCherry
Plasmid#73297PurposeVisualization of Dendritic Golgi SatelliteDepositorInsertsER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
UseTagsER export and mCherryExpressionMammalianMutationPromoterCMV immediate early promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sp_PE
Plasmid#212038PurposeBacterial expression of the PE2 proteins for genome editing in E. coliDepositorInsertPE2
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only