Addgene

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pcDNA3.1(+) EGFP-RalA(WT)

Plasmid

#224274

Purpose

Cell transfection and expression of RalA

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RalA (RALA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV4_PrME

Plasmid

#207266

Purpose

Mammalian expression of Dengue virus 4 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV4_PrME (POLY Dengue virus 4)

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis and TEV site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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ORFtag_TetR-SD_Frame1

Plasmid

#220981

Purpose

ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

Depositor

Alexander Stark

Insert

TetR repressor

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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4X PRE TK luc

Plasmid

#206159

Purpose

Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

4X PRE TK luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cre

Plasmid

#34564

Purpose

Retroviral expression of Cre

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pFLP-hyg

Plasmid

#87831

Purpose

Expresses FLP recombinase with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Use

Tags

None

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-EF1-Cre-PGK-Puro

Plasmid

#108543

Purpose

Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter

Depositor

Javier Alcudia

Inserts

cre recombinase
Puromycin

Use

Tags

Expression

Mutation

Promoter

EF1apha and PGK

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBT376

Plasmid

#125617

Purpose

Expresses BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-hISG15

Plasmid

#12447

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15 fragment (ISG15 Human)

Use

Tags

Expression

Mammalian

Mutation

ISG15 up to LRLRGG

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pRDA_886

Plasmid

#201162

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCitrine

Plasmid

#188386

Purpose

Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

Gal4UAS_tBFP_PGK_mCitrine

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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