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pCBASceI

Plasmid

#26477

Purpose

I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

Depositor

Maria Jasin

Article

Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

Insert

pCBASceI

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132H

Plasmid

#81686

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R132H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDESTsplice

Plasmid

#32484

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11

Plasmid

#221183

Purpose

cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PQ5 (cumate-inducible)

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-N2cG

Plasmid

#100811

Purpose

express rabies virus CVS N2c strain glycoprotein gene (N2cG)

Depositor

Ian Wickersham

Insert

rabies virus CVS N2c strain glycoprotein gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-CD81-10

Plasmid

#55012

Purpose

Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CD81 (CD81 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-neo-CD2-FAK

Plasmid

#37013

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

CD2-FAK (CD2, PTK2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

Plasmid

#138150

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Tags

Expression

Mutation

DR1 A>T

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-FLAG-CAMK2D

Plasmid

#221699

Purpose

Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAR17_StrepI_hFASh_H8_pET22b

Plasmid

#122846

Purpose

Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

human FASN/ human fatty acid synthase (FASN Human)

Use

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET28c-F30-Broccoli

Plasmid

#66788

Purpose

Expresses Broccoli in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Broccoli in the F30 scaffold

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 D210N

Plasmid

#174448

Purpose

Express GFP-tagged RNase H1 D210N in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Use

Tags

His-tag, GFP

Expression

Bacterial

Mutation

D210N

Promoter

T7 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMIG-FLAG-MLL-AF9

Plasmid

#71443

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-Af9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-hSyn-RFP

Plasmid

#22909

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Has Service

Cloning Grade DNA

Insert

human synapsin I promoter driving DsRedExpress (SYN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2010

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Phusion

Plasmid

#206210

Purpose

Inducible expression of DNA polymerase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

Phusion DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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ph7SK-gRNA

Plasmid

#53189

Purpose

Expresses the S. pyogenes sgRNA from the 7SK promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human 7SK

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human H1

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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