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pTW081

Plasmid

#115929

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/3 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW082

Plasmid

#115930

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW083

Plasmid

#115931

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/14 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP V107M-L273D-L276D

Plasmid

#114183

Purpose

ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

V107M/L273D/L276D

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP T184M-L273D-L276D

Plasmid

#114184

Purpose

ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

T184M/L273D/L276D

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDONOR-PARK6e5-I368N

Plasmid

#86154

Purpose

Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

PINK1 exon 5 homology arms (PINK1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 R419A/K422A/K426A

Plasmid

#111793

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R419A/K422A/K426A

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 L373A/N376A/D385A/S387A

Plasmid

#111789

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L373A/N376A/D385A/S387A

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 R404A/D406A/D407A

Plasmid

#111790

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R404A/D406A/D407A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 D409A/G411A/Q415A

Plasmid

#111791

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D409A/G411A/Q415A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104619

Purpose

Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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PatoM-OG241

Plasmid

#72842

Purpose

Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

Depositor

Chris Barnes

Article

Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

Insert

PatoM

Use

Tags

Expression

Bacterial

Mutation

Promoter

PatoM

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LacI-11/109-pHG165c

Plasmid

#90059

Purpose

Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI-11/109

Use

Tags

Expression

Bacterial

Mutation

109

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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CDk4-luciferase fragment B

Plasmid

#86659

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

generated by cleavage at the KpnI site from 880 (…

Promoter

CDK7

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4F07

Plasmid

#71056

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Mi-2 (Mi-2 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1G05

Plasmid

#70809

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIbeta (CkIIbeta Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B07

Plasmid

#70819

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CkIIalpha (CkIIalpha Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160E,NoCys)

Plasmid

#66875

Purpose

Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations

Use

Tags

Histag

Expression

Bacterial

Mutation

S160E; changed 4 (all) cysteines to serine

Promoter

T7lac

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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