Addgene

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pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pmApple-EGFP-HRas

Plasmid

#133854

Purpose

Mammalian expression of HRas N-terminally tagged with mApple and EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Use

Tags

EGFP and mApple

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJB557

Plasmid

#115967

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP 2xTetApt-STOP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJB554

Plasmid

#115964

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP 2xTetApt-STOP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW101

Plasmid

#115944

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP 2x K-turn

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTW098

Plasmid

#115941

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 2x K-turn

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW097

Plasmid

#115940

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/3 SGP 2x K-turn

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW095

Plasmid

#115938

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/16 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW084

Plasmid

#115932

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/15 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1beta

Plasmid

#124140

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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rat brain inositol 1,4,5-trisphosphate 3-kinase A

Plasmid

#122155

Purpose

rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGDF11 #2

Plasmid

#83084

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Tags

Expression

Mutation

a mutated form of FLP, which at position 5 contai…

Promoter

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC Glu39Ala_P2A_Hygro_Barcode

Plasmid

#120512

Purpose

Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC Glu39Ala (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Point mutation changing Glutamic Acid to Alanine …

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 CR3m shRNA

Plasmid

#120795

Purpose

Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 UTR3m shRNA

Plasmid

#120797

Purpose

Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJB553

Plasmid

#115963

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 1xTetApt-STOP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJB555

Plasmid

#115965

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP 2xTetApt-STOP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW096

Plasmid

#115939

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/30 SGP 2x K-turn

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW100

Plasmid

#115943

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/15 SGP 2x K-turn

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge