pmApple-EGFP-HRas
(Plasmid
#133854)
-
PurposeMammalian expression of HRas N-terminally tagged with mApple and EGFP
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 133854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneC3
-
Backbone manufacturerClontech
- Total vector size (bp) 6037
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameHRas
-
Alt nameH-Ras
-
SpeciesH. sapiens (human)
-
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
-
Tags
/ Fusion Proteins
- mApple (N terminal on insert)
- EGFP (N terminal on insert)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byAnne Kenworthy
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pmApple-EGFP-HRas was a gift from Dave Piston (Addgene plasmid # 133854 ; http://n2t.net/addgene:133854 ; RRID:Addgene_133854) -
For your References section:
Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins. Foust DJ, Godin AG, Ustione A, Wiseman PW, Piston DW. Biophys J. 2019 Nov 5;117(9):1764-1777. doi: 10.1016/j.bpj.2019.09.028. Epub 2019 Sep 28. 10.1016/j.bpj.2019.09.028 PubMed 31606123