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Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC5
Plasmid#183200PurposeEntry cloneDepositorInsertCas9-RFC5
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ctdnep1_Cter
Plasmid#196522PurposeEntry vector for Gateway with Ctdnep1_CterDepositorInsertCtdnep1-Cter (CTDNEP1 Human)
UseTagsExpressionBacterialMutationNo transmembrane domainPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Ctdnep1
Plasmid#196520PurposeEntry vector for Gateway with Ctdnep1DepositorInsertCtdnep1-N1 (CTDNEP1 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-CDC42-DN
Plasmid#109584PurposeMultisite gateway vector for 3' tagging with dominant negative CDC42DepositorInsertCDC42 (cdc42 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-N (TRP1)
Plasmid#177797PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX-CFP-STIM1-deltaK
Plasmid#18860DepositorInsertSTIM1 (STIM1 Human)
UseTagsCFPExpressionMammalianMutationStop codon at amino acid position 671 of STIM1. C…PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-CnVA
Plasmid#24587DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLIK Zeo p53DD
Plasmid#136534PurposeLentiviral expression vector for an inducible p53DDDepositorInsertp53DD (Trp53 Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationThis construct was created from the pBabe p53DD (…PromoterAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMYH9-D1424H-FLAG
Plasmid#183513PurposeGateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.DepositorInsertMYH9 (MYH9 Human)
UseTagsFLAGExpressionMammalianMutationchanged Aspartic acid 1424 to HistidinePromoterCMVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A14
Plasmid#132105PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A14 (SLC39A14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
P1_Firre_1.49_pDONR221
Plasmid#195204PurposePlasmid with pDONR221 backbone containing Firre 1.49 clone. Kanamycin resistance. Gateway cloning system.DepositorInsertmFirre 1.49 Clone
UseGateway cloning intermediateTagsExpressionMutationOnly exonsPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9
Plasmid#87917PurposeGateway cloningDepositorInsertsgHTT1, sgGFP2, shCas9
UseTagsExpressionMammalianMutationPromoterH1, U6, 7SKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A3
Plasmid#132301PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A3 (SLC7A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV 3xFLAG-ATG3 K243R
Plasmid#129301PurposeExpresses pCMV 3xFLAG-ATG3 K243R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 243 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-C (LEU2)
Plasmid#177796PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[3]ExpressionYeastMutationPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-C (TRP1)
Plasmid#177795PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-iRFP670 L1-R5
Plasmid#84390PurposeMultisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsiRFP670ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-HMGB1
Plasmid#183198PurposeEntry cloneDepositorInsertCas9-HMGB1
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only