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Addgene

P1_Firre_1.49_pDONR221
(Plasmid #195204)

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Item Catalog # Description Quantity Price (USD)
Plasmid 195204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDONR221
  • Backbone size w/o insert (bp) 4761
  • Total vector size (bp) 7461
  • Vector type
    Gateway Cloning Intermediate

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The Rinn Lab uses Omnimax 2T1R cells for transformation.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mFirre 1.49 Clone
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    Only exons
  • GenBank ID
    103012

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P1_Firre_1.49_pDONR221 was a gift from John Rinn (Addgene plasmid # 195204 ; http://n2t.net/addgene:195204 ; RRID:Addgene_195204)

  • For your References section:

    Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre. Hacisuleyman E, Goff LA, Trapnell C, Williams A, Henao-Mejia J, Sun L, McClanahan P, Hendrickson DG, Sauvageau M, Kelley DR, Morse M, Engreitz J, Lander ES, Guttman M, Lodish HF, Flavell R, Raj A, Rinn JL. Nat Struct Mol Biol. 2014 Feb;21(2):198-206. doi: 10.1038/nsmb.2764. Epub 2014 Jan 26. 10.1038/nsmb.2764 PubMed 24463464
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