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Plasmid#204203PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3 and eGFPExpressionMammalianMutationGly to Ala 1134, 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halotag-6xHp
Plasmid#214414PurposeLipid droplet localization of 6xHp of Spastin fused to HalotagDepositorInsert6x Spastin aa 43-92 (SPAST Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-6xHp
Plasmid#214416PurposeLipid droplet localization of CFAST fused to Halotag-6xHpDepositorInsertdeletion
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mApple-1xHp
Plasmid#214409PurposeLipid droplet and ER localization of 1xHp of Spastin fused to mAppleDepositorInsertSpastin aa 43-92 (SPAST Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN
Plasmid#206967PurposeExpresses TadA7.10 and SpG cas9N by the constitutive CMV promoterDepositorInsertCMV, TadA7.10, SpG N
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-TadA8e-SpG N aa2-713-InteinN
Plasmid#206968PurposeExpresses TadA8e and SpG cas9N by the constitutive CMV promoterDepositorInsertCMV, TadA8e, SpG N
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Lmna sgRNA
Plasmid#206979PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoterDepositorInsertLmna sgRNA
UseAAV; Adenine base editorTagsExpressionMutationPromoterU6Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold
Plasmid#208111PurposeExpresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoterDepositorInsertSpG C, U6, scaffold
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Creb5(HA)
Plasmid#195022PurposeGateway vector containing HA-tagged Creb5DepositorInsertHA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
UseGateway cloningTags3xHAExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 E2E1- Prg4 promoter-luciferase
Plasmid#195035PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10 E3E2E1-Prg4 promoter-luciferase
Plasmid#195036PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10 E4E3E2E1-Prg4 promoter-luciferase
Plasmid#195037PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertpGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’eGFP/sgLMNA
Plasmid#170546PurposeExpresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology armsDepositorInsertsgRNA targeting 5'- end of LMNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV.5’eGFP/sgVIM
Plasmid#170548PurposeExpresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology armsDepositorInsertsgRNA targeting 5'- end of VIM
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f L27A
Plasmid#204138PurposeProduction of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coliDepositorInsertjGCaMP8f L27A
UseTagsN-terminal His tagExpressionBacterialMutationLeucine 27 changed to AlaninePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f L27A
Plasmid#204140PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutationDepositorInsertjGCaMP8f L27A
UseTagsN-terminal His tagExpressionMammalianMutationLeucine 27 changed to AlaninePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 genomic A. thaliana PLDGAMMA3
Plasmid#209188PurposepENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tagsDepositorInsertAT4G11840 (PLDGAMMA3 Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA
Plasmid#174897Purposeochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassetteDepositorInsertsf GFP
UseTagsExpressionMammalianMutation150TAA in sfGFPPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA
Plasmid#174898Purposeopal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassetteDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TGA in sfGFPPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-mSox2C17
Plasmid#206369PurposeExpresses mouse SOX2-C17 in mammalian cells, for lentivirus generation.DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only