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Plasmid#140738PurposeLentiviral vector to overexpress human PTEN and GFPDepositorInsertGFP-PTA-PTEN (PTEN Human)
UseLentiviralTagsnoneExpressionMammalianMutationPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationK842M mutation (Catalytic Inactive)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-hESRRB
Plasmid#60434PurposeOver-expression of human ESRRBDepositorInsertESRRB (ESRRB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266E
Plasmid#161521PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editingDepositorInsertzSpRYCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…Tags3X FLAG, NLSExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSSCP
Plasmid#183188PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under TDepositorInsertsDivIVA and superfolder GFP fusion protein
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265F2
Plasmid#164722PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)NG-UGI
UseCRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-hCMV1-hKLF2-IV
Plasmid#60433Purposetet-inducible human KLF4DepositorInsertKLF2 (KLF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTq2-Calmodulin
Plasmid#198200PurposeFRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2DepositorInsertCalmodulin 2 (Calm2 Rat)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-ORAI1-EC-HA
Plasmid#174335Purposeencodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).DepositorInserthuman Orai1 (ORAI1 Human)
UseTagsGFP in the N-terminus and HA tag in the extracell…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only