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pPBpuro-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#184717

Purpose

iRaf

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF1-V5-miniTurbo

Plasmid

#207810

Purpose

C-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF1-V5-miniTurbo (NFE2L1 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF1

Plasmid

#207809

Purpose

N-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF1 (NFE2L1 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF3-V5-miniTurbo

Plasmid

#207816

Purpose

C-terminal tagged NRF3 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF3-V5-miniTurbo (NFE2L3 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746

Plasmid

#207819

Purpose

N-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSSA-eGFP (pXK-221)

Plasmid

#208826

Purpose

The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).

Depositor

Kun Xu

Article

Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-ZNF746-V5-miniTurbo

Plasmid

#207820

Purpose

C-terminal tagged ZNF746 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746-V5-miniTurbo (ZNF746 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746-C571A

Plasmid

#207821

Purpose

N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746-C571A (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

C571A

Promoter

UBC

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3-LN

Plasmid

#207129

Purpose

Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA and Lambda Peptide (LN)

Expression

Mutation

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_E210A

Plasmid

#204475

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B E210A mutant

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

E210A

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_R153X

Plasmid

#204476

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B R153X mutant

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

R153X

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_V748L

Plasmid

#204477

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B V748L mutant

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

V748L

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_E993A

Plasmid

#204479

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B E993A mutant

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

E993A

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_I1038T

Plasmid

#204480

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B I1038T mutant

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

I1038T

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

Plasmid

#204621

Purpose

Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro

Plasmid

#202800

Purpose

Lentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFalpha (with intron)

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA1

Plasmid

#201622

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA2

Plasmid

#201623

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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