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Plasmid#111061PurposeExpresses nLight3.1 in mammalian cellsDepositorInsertnLight3.1
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SNAP-CRY2-VHH(GFP)
Plasmid#58370PurposeFor use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MPDepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsSNAP and vhhGFP4ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tec-SH2
Plasmid#46522DepositorInsertTec (TEC Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 236-351PromoterTacAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181a-5p
Plasmid#103276PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC299 VEGF Site#2
Plasmid#47506Purposehuman gRNA expression vector targeting VEGFDepositorInsertgRNA-VEGF site 2 (VEGFA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
LLP469 pEF1a-mCherry-EMPTY-gRNA
Plasmid#100958PurposemCherry driven by EF1a, empty gRNA scaffold driven by U6DepositorInsertgRNA empty backbone with RFP
UseCRISPRTagsRFPExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pRc/CMV
Plasmid#22044DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 98 from Tyrosine to H…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF Su9‐roGFP2‐Tsa2ΔCR
Plasmid#83240PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.DepositorInsertSu9-roGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCr with Su9 targetting se…ExpressionYeastMutationPromoterTEFAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pyc111m
Plasmid#158779Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6m-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTBL437 AAVS1 EQR sgRNA
Plasmid#126447PurposeTo cut the human AAVS1 locus for integration.DepositorInsertspacer against human AAVS1 safe-harbor locus with NGAG PAM
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2a2(apf)
Plasmid#13761DepositorInsertJnk2a2(apf) (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif THr(183)-Pro-T…PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(Y40C)
Plasmid#164903PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.DepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationY40C mutation in the Rac1 domain whcih make the s…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro
Plasmid#192684PurposeLentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNAs #1,2,3 (IL1RN Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES-hrGFP II-mTET1_CD
Plasmid#83571PurposeExpresses a mutant catalytic domain of TET1 in mammalian cellsDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseTags3X FLAG tag and hr GFP IIExpressionMammalianMutationTET1 amino acids 1-1417 deleted, catalytic domain…PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter luciferase delta MEF
Plasmid#8889DepositorInsertPGC-1 alpha promoter dMEF (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationSite-directed mutagenesis to remove the MEF2 bind…PromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
RNAseq_cal_Tuschl
Plasmid#59426PurposeEncodes sequence non-cognate to human genome for generation of RNAseq calibratorsDepositorInsertRNAseq_cal_Tuschl
UseTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDendra2-Hygro
Plasmid#202407PurposeDendra2 expression plasmidDepositorInsertHygromycin
UseTagsDendra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-516b-5p
Plasmid#103601PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-516b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
inhibin-DsRed
Plasmid#156184PurposeExpression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.DepositorInsertsrainbow trout inhibin α promoter
rainbow trout β actin 3'UTR
UseTagsExpressionBacterialMutationPromoterinhibinAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only