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Addgene

pL-CRISPR.SFFV.PAC
(Plasmid #57829)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 57829 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLKO.005
  • Total vector size (bp) 13615
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SpCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4300
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Sp sgRNA scaffold
  • Insert Size (bp)
    75

Gene/Insert 3

  • Gene/Insert name
    SFFV
  • Alt name
    Spleen Focus Forming Virus promoter
  • Species
    Synthetic
  • Insert Size (bp)
    415

Gene/Insert 4

  • Gene/Insert name
    P2A-PAC
  • Alt name
    Puromycine resistance gene
  • Species
    Synthetic
  • Insert Size (bp)
    750

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains the 1.9kb stuffer from the pLKO.005 vector backbone between the BsmBI sites used for sgRNA cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL-CRISPR.SFFV.PAC was a gift from Benjamin Ebert (Addgene plasmid # 57829 ; http://n2t.net/addgene:57829 ; RRID:Addgene_57829)
  • For your References section:

    Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. 10.1038/nbt.2951 PubMed 24952903