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Showing: 6601 - 6620 of 14407 results

  2. pUC57-Amp_CRISPIE donor B5

    Plasmid
    #172846
    Purpose
    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172846-plasmid-map-sequence-id-340979

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  4. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

    Plasmid
    #154769
    Purpose
    plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y125A
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154769-plasmid-map-sequence-id-309855

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  8. pscAAV-Sox2-mStr

    Plasmid
    #135617
    Purpose
    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    P2A-mStrawberry
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135617-plasmid-map-sequence-id-263111

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  9. pC-HYG-YR

    Plasmid
    #61765
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61765-plasmid-map-sequence-id-112551

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  10. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138143-plasmid-map-sequence-id-275400

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  16. DDX41-GFP

    Plasmid
    #175494
    Purpose
    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.
    Depositor
    Evan Braunstein
    Article
    Li et al Haematologica. 2016 Jun;101(6):e228-31.
    Insert
    DDX41 (DDX41 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175494-plasmid-map-sequence-id-345045

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  19. pRMC2-sec:msfGFP

    Plasmid
    #194914
    Purpose
    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci
    Depositor
    Rikke Meyer
    Article
    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.
    Insert
    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    Use
    Tetracycline-inducible expression vector for stap…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194914-plasmid-map-sequence-id-384625

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  20. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid
    #192443
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192443-plasmid-map-sequence-id-379076

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Showing: 6601 - 6620 of 14407 results