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Plasmid#86490Purposemamalian cell expression of FGFR1cDepositorInsertFgfrR1 IIIc 15D8 (Fgfr1 Mouse)
UseTagsExpressionMammalianMutationPromoterMoloney murine leukemia virus LTRAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-60 Grx1-roGFP2-His
Plasmid#64799PurposeBacterial expression of His-tagged Grx1-roGFP2DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsHis and roGFP2ExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-LaminB1-10
Plasmid#58020PurposeLocalization: Nuclear Envelope, Excitation: 555, Emission: 584DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sox2-CreERT2
Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2ExpressionMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tollip M240A,F241A
Plasmid#178051PurposeGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsGFPExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE
Plasmid#138146PurposeExpresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caacDepositorInsertPspCas13b
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10316:GABR1
Plasmid#157850PurposeHA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expressionDepositorInsertGABR1 (GABBR1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194283PurposeEF1a-dCas9-KRAB-GFP with Slc1a3 sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA
Plasmid#205316PurposeAll-in-one AAV vector expressing ISAam1 TnpB and its reRNADepositorInserthumanized ISAam1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-ch-TOG-His6
Plasmid#69108PurposeFor bacterial expression of recombinant human ch-TOG tagged with MBP and His.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)
Plasmid#115789PurposeExpresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.DepositorInsertNDP (NDP Human)
UseTagsalkaline phosphatase and flagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP469
Plasmid#65771PurposeHuman expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
MT363
Plasmid#65858PurposeThe gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.DepositorInsertnahG
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD3 Flag
Plasmid#74895PurposeExpress COMMD3 in mammalian cellsDepositorInsertCOMMD3 (COMMD3 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGW_GPHN.FingR-YFP
Plasmid#46298DepositorInsertGPHN.FingR (GPHN Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-PAK1-AID-ires-EGFP
Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
Plasmid#90015PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Ago2
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only