Addgene

We narrowed to 14,473 results for: LEA

Showing: 641 - 660 of 14473 results

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pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

his6-flag

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_sortase_his

Plasmid

#210406

Purpose

For expression of VHH with C-terminal sortase and his tags

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

sortase-his6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his_myc

Plasmid

#210526

Purpose

For expression of VHH with C-terminal his and myc tags

Depositor

Ray Owens

Article

Eyssen et al Bio-protocol 14(6): e4962.

Type

Empty backbone

Use

Tags

His6-myc

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCA1.339

Plasmid

#203951

Purpose

Spectinomycin-based RSF1010 acceptor Level 1

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.422

Plasmid

#203946

Purpose

Level 0 part. Rhamnose-inducible rhaS_PrhaBAD promoter.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

rhaS-PrhaBAD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.393

Plasmid

#203949

Purpose

Level 0 part. Gentamicin resistance cassette in CDS2 position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

GmR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAT.482

Plasmid

#203952

Purpose

Spectinomycin-based RSF1010 acceptor Level T

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCA1.327

Plasmid

#203953

Purpose

Level 1 Position 1 pUC19 acceptor vector

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.394

Plasmid

#203954

Purpose

Level 0 part. theophylline responsive riboswitch E* promoter.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

PtrcE*

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLN-AIDA-Tyr1

Plasmid

#192831

Purpose

IPTG inducible AIDA-Tyrosinase expression

Depositor

André Studart

Article

Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

Insert

AIDA-Tyr1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pL-lac

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pC1.509

Plasmid

#203955

Purpose

Level 1 self-replicating vector containing a DAPG inducible FnCas12a

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

PphlF-Cpf1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCA0.421

Plasmid

#203950

Purpose

Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.423

Plasmid

#203947

Purpose

Level 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

phlF-PphlF

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDD317 (Pmyo-2::GFP + SEC)

Plasmid

#73344

Purpose

SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

Insert

Pmyo-2::GFP + SEC

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

myo-2

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.425

Plasmid

#203944

Purpose

Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a UP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.426

Plasmid

#203945

Purpose

Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

Cas12a DOWN

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.391

Plasmid

#203948

Purpose

Level 0 part. Cas12a coding sequence from Francisella novicida in CDS1 position

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

FnCas12a

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.385

Plasmid

#203936

Purpose

Up Flank. Neutral site. Up flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

desB UP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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