Skip to main content
Addgene
Showing: 1601 - 1650 of 147948 results

  2. pConRef-2H12.D11

    Plasmid
    #221151
    Purpose
    cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221151-plasmid-map-sequence-id-437168

    Enlarge

  3. tFucci(CA)5

    Plasmid
    #153521
    Purpose
    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.
    Insert
    tFucci(CA)5.
    Use
    Tags
    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    153521-plasmid-map-sequence-id-308996

    Enlarge

  7. AAV-CamKIIa-jGCaMP8m-WPRE

    Plasmid
    #176751
    Purpose
    AAV-mediated expression of GCaMP8m under the CamKIIa promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176751-plasmid-map-sequence-id-349058

    Enlarge

  13. pOpen-lambda red operon

    Plasmid
    #165521
    Purpose
    Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    lambda red operon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165521-plasmid-map-sequence-id-338838

    Enlarge

  16. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

    Enlarge

  27. pMN-333

    Plasmid
    #195467
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195467-plasmid-map-sequence-id-384352

    Enlarge

  31. pCMV-15F11-HA-mCh

    Plasmid
    #129591
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129591-plasmid-map-sequence-id-254269

    Enlarge

  36. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Use
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

    Enlarge

  38. pCMV-15F11-HA-mEGFP

    Plasmid
    #129590
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129590-plasmid-map-sequence-id-254270

    Enlarge

  40. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

    Enlarge

  44. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    133962-plasmid-map-sequence-id-265771

    Enlarge

  50. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

    Enlarge

Showing: 1601 - 1650 of 147948 results