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pcDNA3.1 myc-RNF114

Plasmid

#58295

Purpose

mammalian expression of myc tagged RNF114

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

Plasmid

#176745

Purpose

Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FNIP2

Plasmid

#72295

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
CBE-T1.52

Plasmid

#193285

Purpose

Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.52

Use

Tags

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

Plasmid

#176743

Purpose

Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Cx43-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Chk1-Flag

Plasmid

#22894

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Chk1 (CHEK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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SPLINTR mCHERRY v1 backbone

Plasmid

#179778

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Use

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLX303-ABI1-dCas9

Plasmid

#154474

Purpose

Expresses ABI1 fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ABI1 (ABI1 Mustard Weed)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Includes ABI1 aa 126-423, D143A

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNESx2-EGFP-C1-PABDx2-Spo20

Plasmid

#220082

Purpose

PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

Spo20 (SPO20 Budding Yeast)

Use

Tags

X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

Expression

Mammalian

Mutation

amino acids 51-91, tandem dimer

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJBEI-6411

Plasmid

#47050

Purpose

BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase

Use

Synthetic Biology; Bglbrick

Tags

Expression

Bacterial

Mutation

Promoter

Pbad

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-eLACCO2.1-NGR

Plasmid

#208023

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-Itch/2793

Plasmid

#11434

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

Itch (Itch Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #1 GFP

Plasmid

#106950

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Article

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Use

Tags

6His, TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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MigR1-AE9a

Plasmid

#12433

Depositor

Dong-Er Zhang

Article

Yan et al Nat Med. 2006 Aug . 12(8):945-9.

Insert

AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

AML-ETO protein with ETO exon 9a.

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-mCherry

Plasmid

#159742

Purpose

Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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