Addgene

We narrowed to 14,550 results for: LEA

Showing: 6541 - 6560 of 14550 results

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GFP-RanV19 E1RA2

Plasmid

#118317

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Inserts

RanV19 (RAN Human)
EPac1RA2 (RAPGEF3, RAPGEF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

in-frame insertion of RanV19 into GFPEpac1RA2 and…

Promoter

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)

Plasmid

#110145

Purpose

Transformation and expression of Csy4 and cogGFP proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Tags

cogGFP

Expression

Bacterial and Plant

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-DroshaS302D

Plasmid

#62529

Purpose

Serine 302 of Drosha is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to aspartic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to aspartic acid

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300E

Plasmid

#62528

Purpose

Serine 300 of Drosha is mutated to glutamic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

G168D,Y169D

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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zgc:153086_R (OZ512)

Plasmid

#27187

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:153086

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
LCV2 V-2

Plasmid

#84805

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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LCV2 V-3

Plasmid

#84806

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 NDUFS6(5’UTR)-FF

Plasmid

#85488

Purpose

Firefly luciferase under the control of NDUFS6 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of NDUFS6 (NDUFS6 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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zgc:153086_L (OZ511)

Plasmid

#27186

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zgc:153086

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEMS1210

Plasmid

#29107

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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SDC3_L (OZ595)

Plasmid

#35235

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting SDC3

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
SDC3_R (OZ596)

Plasmid

#35236

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting SDC3

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-F-box:gs10:FRB:HA

Plasmid

#228531

Purpose

F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

F-box:gs10:FRB:HA (SKP2 Synthetic, Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Rbx1:gs10:FRB:HA

Plasmid

#228534

Purpose

Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Rbx1:gs10:FRB:HA (RBX1 Synthetic, Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_sgALDH7A1

Plasmid

#218273

Purpose

CRISPR KO plasmid for ALDH7A1 in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsEMX1

Plasmid

#205637

Purpose

reRNA guide targeting Homo sapiens EMX1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsEMX1 (EMX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-ArgiNLS-AausFP1

Plasmid

#220605

Purpose

Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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