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Plasmid#138956PurposeUsed to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv with C terminal epitope tag (kkv Fly)
UseTagsollas and his-6 added to C terminus of protein co…ExpressionInsectMutationPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-kkv-3::NG
Plasmid#138960PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsert3' end of kkv and neon green (kkv Fly)
UseAn editing vector for drosophilaTagsNeon Green fused to C terminus of KkvExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP hSmad4-HomDonor
Plasmid#206044PurposeDonor plasmid for CRISPR knock-inDepositorInsertSmad4 (SMAD4 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15a_AcCas12n
Plasmid#203806Purposep15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.DepositorInsertAcCas12n
UseCRISPRTagsExpressionMutationPromoterrpslAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG165
Plasmid#202584PurposeRNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG161
Plasmid#202580PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG160
Plasmid#202579PurposeRNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG159
Plasmid#202578PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-dErbB4
Plasmid#197357PurposeA knock-out vector for dog ErbB4.DepositorInsertA gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-bleo-ErbB4
Plasmid#197353PurposeA knock-out vector for dog ErbB4.DepositorInsertA gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-dErbB1
Plasmid#197354PurposeA knock-out vector for dog ErbB1.DepositorInsertA gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-dErbB2
Plasmid#197355PurposeA knock-out vector for dog ErbB2.DepositorInsertA gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX458-dErbB3
Plasmid#197356PurposeA knock-out vector for dog ErbB3.DepositorInsertA gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK809
Plasmid#196406PurposeStrong expression of firefly and nanoluc luciferases codon-optimized for S. rosettaDepositorInsertSro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK810
Plasmid#196407PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly controlDepositorInsertSro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK811
Plasmid#196408PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly controlDepositorInsertSro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-ORF29
Plasmid#200028PurposeLentiviral vector for expression of KSHV ORF29DepositorInsertORF29
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg11 [699-800] pHis2
Plasmid#190264PurposeCodon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.DepositorInsertATG11
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-luciferase
Plasmid#198590PurposeGateway Entry CloneDepositorInsertluciferase
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BOLF1
Plasmid#198579PurposeGateway Entry CloneDepositorInsertBOLF1
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only