We narrowed to 15,769 results for: LEA

1. pTER E2F2shRNA human

   Plasmid

   #66884

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 2 (E2F2 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1 Tet

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBiex PKC - Peptide 8 mCit mCer

   Plasmid

   #83563

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PRKCA (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXD4 (murine) MSCV

   Plasmid

   #8549

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Use

   Retroviral

   Tags

   His and T7

   Expression

   Mammalian

   Mutation

   contains T7 and HIS tags from pET; makes protein …

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Lyn-sACt-mCherry

   Plasmid

   #138216

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   Lyn-sACt-mCherry (Adcy10 Rat)
   

   Tags

   N-terminal targeting sequence from Lyn kinase and…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ165

   Plasmid

   #109327

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   Promoter EC1.2 enhancer fused to EC1.1 promoter

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-APPL1-ΔPTB

   Plasmid

   #59768

   Purpose

   Expresses APPL1 with PTB domain deleted

   Depositor

   Donna Webb

   Article

   Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

   Insert

   APPL1 (APPL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 467-709

   Promoter

   CMV

   Available Since

   Oct. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCin4-3xFLAG-MKL1 S454A

   Plasmid

   #19844

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S454A (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 454 to Alanine

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pMT-ubb-cytoBirA-2a-mCherry

   Plasmid

   #79885

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRevTRE MKL1 S449A/T450A/S454A

    Plasmid

    #19847

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Expression

    Bacterial and Yeast

    Promoter

    TDH3

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pColdI-GST-mGTSF1l

    Plasmid

    #214817

    Purpose

    Bacterial expression of GST-tagged mouse GTSF1l

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF1l (Gtsf1l Mouse)
    

    Tags

    6x His and GST

    Expression

    Bacterial

    Promoter

    CspA

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_EE-NG-nCas9

    Plasmid

    #163542

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pClneoMyc MOB1 T12/35A

    Plasmid

    #47938

    Purpose

    expresses Myc tagged human MOB1 containing T12A and T35A mutations

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T12A and T35A

    Promoter

    CMV

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

    Plasmid

    #166413

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    Protection Of Telomeres 1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB deleted 125 a.a

    Promoter

    ef1-a

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

    Plasmid

    #127865

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    deleted the c-terminal 20 amino acids

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xNLS-NLP-cMyc LbaCas12a

    Plasmid

    #182123

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc LbaCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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