Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 13,953 results for: LEA

Clear keywords
Showing: 6521 - 6540 of 13953 results

  1. TBXTA-c021

    Plasmid
    #139758
    Purpose
    Protein expression/crystallography (PDB:5QT0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-N211
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139758-plasmid-map-sequence-id-296541

    Enlarge

  2. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

    Enlarge

  4. cycLuc_SbMVs

    Plasmid
    #119209
    Purpose
    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Publication
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cyclic luciferase with SbMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119209-plasmid-map-sequence-id-231830

    Enlarge

  5. pOCC94

    Plasmid
    #118912
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    MBP, cleavable with 3C protease and eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118912-plasmid-map-sequence-id-232258

    Enlarge

  8. CHD1 (CHD1A-c107)

    Plasmid
    #110253
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CHD1 (CHD1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110253-plasmid-map-sequence-id-212335

    Enlarge

  9. pBad-HPII TAG392

    Plasmid
    #105848
    Purpose
    expresses E. coli HPII catalase with TAG stop codon at site 392 and N-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    hydroperoxidase II catalase
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 392
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105848-plasmid-map-sequence-id-205565

    Enlarge

  12. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

    Enlarge

  14. 5J3E (THYN1)

    Plasmid
    #79524
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    THYN1 (THYN1 Human)
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79524-plasmid-map-sequence-id-150164

    Enlarge

  15. 5J39 (TDRKH)

    Plasmid
    #79523
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD2 (TDRKH Human)
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79523-plasmid-map-sequence-id-150161

    Enlarge

Showing: 6521 - 6540 of 13953 results