We narrowed to 15,791 results for: LEA

1. LRRK2-K1906M

   Plasmid

   #35946

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pLV-EF1a-Itprip-V5

   Plasmid

   #120241

   Purpose

   Expresses V5-tagged Itprip in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Itprip (ITPRIP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Expression

   Plant

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBactin-Tubg1-mNeonGreen

   Plasmid

   #196869

   Purpose

   Expression of the gamma-tubulin fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Tubg1-mNeonGreen (Tubg1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TAF15N-miRFP670-Cry2WT

   Plasmid

   #122440

   Purpose

   Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Lentiviral

   Tags

   Cry2WT and miRFP670

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-208

   Promoter

   SFFV

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFSynW Substance P-pHluorin IRES mRuby3

   Plasmid

   #195699

   Purpose

   Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Tac1 (Tac1 Mouse)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVC297 VEGF Site#1

   Plasmid

   #47505

   Purpose

   human gRNA expression vector targeting VEGF

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-VEGF site 1 (VEGFA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1_VNp-FGF21

   Plasmid

   #182407

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-fgf21 (FGF21 Human)
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PD-1-miSFIT-12-C, 21-G

   Plasmid

   #124683

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVC228 VEGF Site#3

    Plasmid

    #47507

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 3 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid

    #176276

    Purpose

    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. (p)odr-3::GFP::EGL-4

    Plasmid

    #100897

    Purpose

    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

    Depositor

    Noelle L'Etoile

    Article

    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

    Insert

    56-2741: odr-3 promoter
    

    Expression

    Worm

    Promoter

    odr-3

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_UdgX-EE-UdgX-nCas9-RBMX

    Plasmid

    #163565

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRESneo-FLAG/HA Ago2

    Plasmid

    #10821

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    R668H. Additionally, N terminus different from wi…

    Available Since

    Oct. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-DIO-GRAB_g5-HT2m

    Plasmid

    #208722

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT2m
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTER E2F2shRNA human

    Plasmid

    #66884

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 2 (E2F2 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1 Tet

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBiex PKC - Peptide 8 mCit mCer

    Plasmid

    #83563

    Purpose

    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PRKCA (PRKCA Human)
    

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXD4 (murine) MSCV

    Plasmid

    #8549

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Retroviral

    Tags

    His and T7

    Expression

    Mammalian

    Mutation

    contains T7 and HIS tags from pET; makes protein …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only