We narrowed to 12,057 results for: LEA
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Plasmid#208656PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cellsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
ExpressionMammalianPromoterCMVAvailable SinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Halo-Tag
Plasmid#196898PurposeExpression of Halo-Tag. Use as control for Halo-tag fusionsDepositorInsertHalo-Tag
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT3.0mut
Plasmid#208712PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
ExpressionMammalianPromoterCMVAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-NLS-6xHis
Plasmid#207382PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
TagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-MCP1
Plasmid#184341PurposeExpress yeast MCP1 in Expi293F cellsDepositorInsertYeast MCP1 (MCP1 Budding Yeast)
Tags3xFLAG-PreScission cleavage siteExpressionMammalianPromoterCMVAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL6
Plasmid#184134PurposeFor labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-E795L
Plasmid#176890PurposeGateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoterDepositorInsertLbCas12a-E795L
UseCRISPR; Gateway compatible lbcpf1 entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTox-EGFPsite1_NGA-PAM (BPK754)
Plasmid#181747Purposep11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAMDepositorInsertccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
UseSelection plasmidAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem161a-V5
Plasmid#175149PurposeLentiviral expression of mouse Tmem161a-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem53-V5
Plasmid#175142PurposeLentiviral expression of mouse Tmem53-V5DepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mCherry-Vav2cat
Plasmid#178865PurposeMammalian expression of Vav2(DH-PH-CRD) fused to FKBP-mCherryDepositorAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-Dendra2
Plasmid#169216PurposeTargeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locusDepositorInsertDouble SapI flanked Dendra2-P2A-Puro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC0_2_VP64
Plasmid#163714PurposePlasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)DepositorInsertVP64 + SV40 NLS
UseSynthetic Biology; Moclo vector level 0 (type 4a)Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9748-dCBE
Plasmid#163008Purposeplasmid expressing CBE with dCas9DepositorInsertdCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA528-PPxY1-GS-WW3e
Plasmid#166705Purposeexpresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cellsDepositorInsertAMOT PPxY1 fused to NEDD4L WW3
TagsHis-SUMOExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a Bm-iPGM-bio
Plasmid#162565PurposeExpresses B. malayi iPGM containing a C-terminal biotinylation site and His tagDepositorInsertB. malayi iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-NARS-2
Plasmid#166532PurposescFv of a human scaffold targeting Asparaginyl-tRNA synthetase. Antigen coverage aa 1-458 of 458, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-AARS-51
Plasmid#166539PurposescFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-455 of 968DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only