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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3b

Plasmid

#111056

Purpose

Expresses dLight1.3b in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3b

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a-dCas9-KRAB-GFP backbone

Plasmid

#194281

Purpose

Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

Plasmid

#138148

Purpose

Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NES

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286D)

Plasmid

#127391

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Aspartic acid

Promoter

pCAG

Available since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154786

Purpose

Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

Depositor

Robert Singer

Article

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Fly, Synthetic)

Use

Lentiviral

Tags

GFP, V5, and mCherry

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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LifeAct-14 (LA-14)

Plasmid

#158750

Purpose

Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

Depositor

Minhajuddin Sirajuddin

Article

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

LifeAct-14

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Deletion mutation for last three amino acids from…

Promoter

CMV immediate early

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Caveolin-C-10

Plasmid

#55008

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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OMMGFP 1-10

Plasmid

#164119

Purpose

Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

OMMGFP 1-10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5A-SH2

Plasmid

#46515

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5A (STAT5A Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 582-693

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-SQSTM1

Plasmid

#223715

Purpose

Expresses Flag-tagged SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-TurboID-SQSTM1

Plasmid

#223714

Purpose

Expresses Flag-tagged TurboID SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-EBFP-tTAn

Plasmid

#172122

Purpose

Constitutive expression of EBFP and rtTA-InteinN

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126216

Purpose

Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Hsa

Plasmid

#128175

Purpose

Doxycyclin inducible shRNA knockdown of human cGAS gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Article

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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