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Showing: 6381 - 6400 of 21945 results

  1. CoV2-N-A119S

    Plasmid
    #177948
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (A119S) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A119S
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177948-plasmid-map-sequence-id-348195

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  6. pJH4260

    Plasmid
    #191678
    Purpose
    Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    GCaMP6s
    Use
    Tags
    wScarlet
    Expression
    Worm
    Mutation
    Promoter
    Ptwk-40
    Available since
    Nov. 30, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    191678-plasmid-map-sequence-id-375288

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  7. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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  8. pJH2685

    Plasmid
    #191372
    Purpose
    Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Tetanus Toxin Light Chain
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Punc-4
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191372-plasmid-map-sequence-id-377546

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  13. pHDR-eGFP (pXK-1647)

    Plasmid
    #208824
    Purpose
    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208824-plasmid-map-sequence-id-417244

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  15. HypaCas9

    Plasmid
    #138557
    Purpose
    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    HypaCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138557-plasmid-map-sequence-id-274767

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  16. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  18. ROSA26(MultiFPsΔPuro)

    Plasmid
    #140759
    Purpose
    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
    Depositor
    Hideyuki Okano
    Article
    Serizawa et al Development. 2019 Nov 4;146(21). pii: de
    Inserts
    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    June 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140759-plasmid-map-sequence-id-311014

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Showing: 6381 - 6400 of 21945 results