These results may also be relevant.
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pcDNA3.1-Cerulean-Epac2(L426W)-Venus
Plasmid#138200PurposeFRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
UseTagsCerulean and VenusExpressionMammalianMutationContains leucine-to-tryptophan substitution at re…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
OptoProfilin
Plasmid#208286PurposeExpresses Cry2(1-531)-mCherry-Profilin.WTDepositorUseTagsmCherryExpressionMammalianMutationProfilin WT (Mus musculus)PromoterCMVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGES201
Plasmid#190197PurposeFor CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro Tusc5
Plasmid#87952Purposeretroviral plasmid containing mouse Tusc5DepositorInsertTumor Suppressor Candidate 5 (Tusc5 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
VAMP3-mCherry
Plasmid#92423PurposeExpression of VAMP3 C-terminally conjugated to mCherry.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5C-FLAG
Plasmid#72196PurposeExpresses full-length Unc5c with a C-terminal FLAG tagDepositorInsertUnc5c (Unc5c Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2
Plasmid#89735PurposeExpresses wildtype mouse TET2 in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-ADGRL3-Flag
Plasmid#201218PurposepCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal FlagDepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-myc-Itch (C822S)/2860
Plasmid#11428DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationcysteine at 822 mutated to serinePromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13B tail 442-1843
Plasmid#134623PurposeMammalian expression of mouse KIF13B 442-1843DepositorInsertKIF13B (Kif13b Mouse)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 441 N-terminal residues.PromoterChicken Beta actinAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag
Plasmid#181814PurposeExpresses murine Muc5b D1 with cleavable His tag, residues 50-426DepositorInsertMuc5b D1 (Muc5b Mouse)
UseTagsTEV cleavage site followed by 6xHistidine tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)
Plasmid#160608PurposeTU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)DepositorInsertP35s:dCas9:SRDX:tNOS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S197A
Plasmid#131522PurposeExpresses MKP-3 with S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 197 to AlaninePromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-Lrat
Plasmid#206345PurposeAAV plasmid expressing LRAT in photoreceptorsDepositorInsertLrat (Lrat Mouse)
UseAAVTagsExpressionMutationPromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.a-Fc-His
Plasmid#72180PurposeExpresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5c.a (Unc5c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADGRL3-CTF-Flag
Plasmid#201219PurposepCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal FlagDepositorInsertADGRL3 (Adgrl3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC71167
Plasmid#154066PurposeLevel 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFPDepositorInsertZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterZea mays Ubiquitin PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-H1-10
Plasmid#55487PurposeLocalization: Nucleus/Histones, Excitation: 462, Emission: 492DepositorInsertH1 (H1f0 Mouse)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)
Plasmid#19746DepositorInsertoligo targeting p53 and PTEN (Pten Mouse)
UseCre/Lox, RNAi, and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only
