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Plasmid#87793PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP5.v1 puro
Plasmid#87792PurposeExpresses an inducible short hairpin targeting human MKP5 sequenceDepositorInsertshMKP5 (DUSP10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pf CAG mc2 IRES puro
Plasmid#206233PurposeFor expressing cloned exons as circular RNA.DepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#32-Tet-pLKO-puro
Plasmid#107399PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-TARDBP-A315T_VA
Plasmid#141329PurposeLentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cellsDepositorInsertTARDBP-A315T (TARDBP Human)
UseLentiviralTagsVA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)ExpressionMammalianMutationA315TPromoterAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg1 (CG509)
Plasmid#139455PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A90V
Plasmid#133754PurposeLentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged alanine 90 to valinePromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shSMAD4 #1
Plasmid#83089PurposeLentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2
Plasmid#112837Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH2 + SH3 (C-term) domains of Grb2PromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN1-C911
Plasmid#125784Purposea seed-matched control for pRSITEP-puro-shWRN1DepositorInsertshWRN1-C911 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 dC
Plasmid#38282DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationTruncate mutant 1-265 amino acids residuesPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA Q75L
Plasmid#19719DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationGTPase deficient & constitutively active Gl…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (Puro) p65 shRNA1
Plasmid#22507DepositorInsertp65 shRNA1 (Rela Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xCas9RA-P2A-Puro
Plasmid#110871PurposeLentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)DepositorInsertxCas9(3.7)RA
UseLentiviralTagsFLAGExpressionMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalB S28N
Plasmid#19722DepositorInsertV-ral simian leukemia viral oncogene homolog B (RALB Human)
UseRetroviralTagsExpressionMammalianMutationS28N for dominant negative function.PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Flag-mMcl-1
Plasmid#32982DepositorInsertMcl-1 (Mcl1 Mouse)
UseRetroviralTags3XFlagExpressionMammalianMutationPromoterLTRAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SFFV-NFE2L18ND-Puro
Plasmid#181918PurposeLentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutation8 putative N-glycoyslation sites converted from a…PromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DHFR.cHSF1
Plasmid#134739PurposeLentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseLentiviralTagsDHFRExpressionMammalianMutationDeletion of amino acids 186-202PromoterCMVAvailable sinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-irf3-shrna5
Plasmid#127649PurposeKnock-down of human IRF3DepositorInsertIRF3 shRNA (IRF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE-T2-∆mIR-IRES-Puromycin
Plasmid#105168PurposeMammalian expression vector for expressing tandem cDNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterTet/CMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-4493NES(Booster-PKA)
Plasmid#184709PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-CLEC16A-EMP2
Plasmid#69468PurposeExpresses CLEC16A-EMP2 fusion geneDepositorUseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN2-C911
Plasmid#125786Purposea seed-matched control for pRSITEP-puro-shWRN2DepositorInsertshWRN2 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro C-Raf 22W
Plasmid#12593DepositorInsertN-term truncated mut of Raf-1 (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutation22W, N-terminally truncatedPromoterAvailable sinceSept. 21, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalB Q72L
Plasmid#19721DepositorInsertV-ral simian leukemia viral oncogene homolog B (RALB Human)
UseRetroviralTagsExpressionMammalianMutationQ72L constitutively active, GTPase DeficientPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E4-T2A-Puro
Plasmid#190810PurposeExpress APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 1
Plasmid#22209DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP1.v1 puro
Plasmid#87790PurposeExpresses an inducible short hairpin targeting human MKP1 sequenceDepositorInsertshMKP1 (DUSP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shSTIM1
Plasmid#89816PurposeKnockdown human STIM1 expressionDepositorInsertSTIM1 shRNA (STIM1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro H-Ras 61L
Plasmid#13485DepositorInsertHRas 61L (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation61LPromoterAvailable sinceDec. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 pBabe puro
Plasmid#13596DepositorInsertintegrin beta 6 (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-Puro-shPP2A-Aalpha
Plasmid#15249DepositorInsertPP2A regulatory subunit A, alpha isoform (PPP2R1A Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shGATA6 puro
Plasmid#72615PurposeExpresses an inducible short hairpin targeting human GATA6 sequenceDepositorInsertshort hairpin GATA binding protein 6
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE TightAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro- MLL3-PRKAG2
Plasmid#69473PurposeExpresses MLL3-PRKAG2 fusion geneDepositorInsertMLL3-PRKAG2 (PRKAG2 Human)
UseRetroviralTagsHA TAG:ExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W
Plasmid#208554PurposeLentiviral vector expressing gRNA targeting human BCL11B-E1DepositorInsertBCL11B-E1(14) (BCL11B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆Nterm-mNeonGreen-IRESpuro2
Plasmid#183740PurposeExpresses Ki67∆Nterm-mNeonGreenDepositorInsertMKI67 LR domain + all Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆FHA-mNeonGreen-IRESpuro2
Plasmid#183739PurposeExpresses Ki67∆FHA-mNeonGreenDepositorInsertMKI67_lacking FHA (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking FHA)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shLuc
Plasmid#83092PurposeLentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)DepositorInsertshLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4-ires-puro
Plasmid#167828PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-hTCAB1-puroR
Plasmid#167459Purpose3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cellsDepositorInsertTCAB1 (WRAP53 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
aMHC-Puro Rex-Blast
Plasmid#21231DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-2A-rtTA-TcVA
Plasmid#24592DepositorInsertGateway(TM) cassette
UseLentiviralTagsVAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMY-mCherry-P2A-PuroR-HA
Plasmid#163342PurposeRetroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection markerDepositorInsertmCherry
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-GFP-NBR1-dUBA
Plasmid#74205PurposeRetroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codonDepositorInsertneighbor of BRCA1 gene 1 (NBR1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationChanged aa 877 to encode for premature stop codonPromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide-Puro/CD73-gRNA4
Plasmid#132588PurposeLentiviral gRNA vector targeting human CD73DepositorInsertCD73 (NT5E Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP
Plasmid#67990PurposeCRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPREDepositorInsertU6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3
Plasmid#160252PurposeFor stable integration of FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only