Skip to main content
Addgene
Showing: 621 - 640 of 1756 results

  1. pCfB2189

    Plasmid
    #67532
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67532-plasmid-map-sequence-id-116416

    Enlarge

  5. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid
    #110568
    Purpose
    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
    Depositor
    Alexander Nectow, Jeffrey Friedman
    Insert
    3xFLAG/HA-tagged anti-GFP VHH
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110568-plasmid-map-sequence-id-213334

    Enlarge

  6. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid
    #185699
    Purpose
    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter
    Depositor
    Lynette Lim
    Inserts
    Tdtomato,
    Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    mDlx
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185699-plasmid-map-sequence-id-366333

    Enlarge

  7. pJW1584

    Plasmid
    #121055
    Purpose
    YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    YPET^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized YPET, and auxi…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121055-plasmid-map-sequence-id-235991

    Enlarge

  14. AAV-KrasG13C/sgKras/Cre

    Plasmid
    #99856
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99856-plasmid-map-sequence-id-193794

    Enlarge

  16. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid
    #113685
    Purpose
    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    inverted de-catalyzed SaCas9
    Use
    AAV, CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    NLS and VP64
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113685-plasmid-map-sequence-id-232027

    Enlarge

  19. pAAV-iCre-2A-cherry

    Plasmid
    #182246
    Purpose
    Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182246-plasmid-map-sequence-id-355958

    Enlarge

  20. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid
    #154827
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    TagBFP
    HygR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154827-plasmid-map-sequence-id-306202

    Enlarge

Showing: 621 - 640 of 1756 results