-
Plasmid#89319Purposeexpression of M1 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCg-C1
Plasmid#112266PurposeITRACKgamma Donor ConstructDepositorInsertprotein kinase c gamma (Prkcg Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn2a-HaloTag-V5-EF1-sfGFP
Plasmid#182564PurposeFor mouse Nav1.2 knockin with HaloTag-V5 at the C-terminalDepositorInsertsmouse Scn2a gRNA and HaloTag-V5 donor DNA
sfGFP
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP/WT SP M85
Plasmid#89317PurposeExpression of mouse GFP/WT spastin M85 in mammalian cellsDepositorInsertSPAST M85 (Spast Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTags(His)6-ThioredoxinExpressionBacterialMutationPromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChR2-mRuby2-ST
Plasmid#109125PurposeCation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChR2-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/M87 C448Y
Plasmid#89320Purposeexpression of M87 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deletedPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH2069
Plasmid#179224PurposePnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherryDepositorInsertUNC-9 (unc-9 Nematode)
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97deltaD2
Plasmid#17229DepositorInsertp97 (Vcp Mouse)
UseTagsHisExpressionBacterialMutationchanged codon 457 to stop codonPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G0-R0
Plasmid#101803PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-E1.1-NRSE-RFP
Plasmid#22929DepositorInsertE1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
FAM19A1-V5
Plasmid#184337PurposeExpression of V5-tagged mouse FAM19A1DepositorInsertFAM19A1 (Tafa1 Mouse)
UseTagsV5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2141
Plasmid#179248PurposePunc-4::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherryDepositorInsertUNC-9 (unc-9 Nematode)
UseTagswCherryExpressionWormMutationPromoterPunc-4Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2037
Plasmid#179220PurposePsra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherryDepositorInsertUNC-7 (unc-7 Nematode)
UseTagswCherryExpressionWormMutationPromoterPsra-11Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-mScn2a-3xV5-EF1-smFP-flag
Plasmid#182561PurposeFor mouse Nav1.2 knockin with 3xV5 at the C-terminalDepositorInsertsmouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag
UseAAV and CRISPRTagsExpressionMutationPromoterEF1 and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV1-EF1α-F-Flex-jGCaMP7b-WPR
Plasmid#171689PurposeFlp-dependent expression of jGCaMP7bDepositorInsertGCaMP7b
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nirButterfly
Plasmid#136590PurposeExpression of a genetically-encoded voltage indicator nirButterflyDepositorInsertnirButterfly
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS15
Plasmid#52652Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS15
UseTagsExpressionMammalianMutationdeleted G47/H48 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Chronos(M140T)-mRuby2-ST
Plasmid#109129PurposeModified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos(M140T)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only