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LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT14.G

Plasmid

#201002

Purpose

pLv1-pUC18R6KT-miniTn7-aad9-sfGFP, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pME-GFP-P2A

Plasmid

#80807

Purpose

GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-P2A

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p1.2-Neo

Plasmid

#162739

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO Elongation factor 1A

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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NTM (FLM-PC1Δ137)

Plasmid

#41572

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

sequence of PKD1 FLM from 4078 - 4273

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Use

Tags

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Promoter

Available since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#176878

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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FLM-PC1Δ30

Plasmid

#41568

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ30; sequence of PKD1 from 4078 - 4273

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ45

Plasmid

#41569

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ45; sequence of PKD1 from 4078 - 4258

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ67

Plasmid

#41570

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ67; sequence of PKD1 from 4078 - 4236

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ103

Plasmid

#41571

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Δ103; sequence of PKD1 from 4078 - 4200

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
CTSP-PC1

Plasmid

#41577

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Use

Tags

2xMyc/His and myc/myc/his

Expression

Mammalian

Mutation

FLS (4172-4303)

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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SI3-Flrt3

Plasmid

#28217

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Flrt3 mouse monoclonal antibody (flrt3 Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAV0327

Plasmid

#133488

Purpose

Expression of nucleo-cytoplasmic sfGFP

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pQadd2

Plasmid

#135662

Purpose

Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Lox511/66 and LoxFas/71

Use

Clostridial vector, pamβ1 origin

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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