A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia.
Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC
mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 10.1128/mSphere.00710-19.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
135655 | pQlox71F | Clostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site. |
135656 | pQlox71R | Clostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site. |
135657 | pQlox66R | Clostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site. |
135658 | pQlox66F | Clostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site. |
135659 | pQcre1 | Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites. |
135660 | pQadd1F | Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site. |
135661 | pQadd1R | Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location. |
135662 | pQadd2 | Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R. |