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Depositing Lab
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Publication
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 28217 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlrt3 mouse monoclonal antibody
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Alt nameflrt3
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SpeciesD. rerio (zebrafish)
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Entrez Geneflrt3 (a.k.a. wu:fb75d02, wu:fb93g11, wu:fc10a05, si:dkeyp-24h12.1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See map and Genbank file for plasmid details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SI3-Flrt3 was a gift from Gavin Wright (Addgene plasmid # 28217 ; http://n2t.net/addgene:28217 ; RRID:Addgene_28217) -
For your References section:
A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357