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Plasmid#191445PurposePrgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPregf-1Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-FLCN
Plasmid#72291PurposeoverexpressionDepositorInsertFLCN (FLCN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICSL11059
Plasmid#68263PurposeLevel 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassetteDepositorInsertPromoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD/Paro-IFN
Plasmid#205439PurposeA donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genesDepositorInsertsdog interferon α-4 gene
paromomycin resistance gene
UseCre/Lox; Chlamydomonas expressionTagsExpressionMutationPromoterHeat shock protein 70A (HSP70A)/ribulose bisphosp…Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-cmv-SpCas9-TadDE-BlastR
Plasmid#193850PurposeExpress TadDE in mammalian cells with BlastR selection, Tol2 integrationDepositorInsertTadDE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-FNIP2
Plasmid#72294PurposeoverexpressionDepositorInsertFNIP2 (FNIP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
INPP5D-eGFP WT
Plasmid#161998PurposeMammalian expression vector, containing a GFP tagged version of INPP5DDepositorInsertINPP5D (INPP5D Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_alphaTubulin_C1
Plasmid#85047PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorInsertAlpha-Tubulin (TUBA1B Human)
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-G1-ATP6-Myc-FLAG (puromycin)
Plasmid#86851Purposemammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tagsDepositorInsertATP6 (ATP6 Human)
UseTagsATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…ExpressionMammalianMutationcodon correctedPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sniper-Cas9
Plasmid#138559PurposeExpresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSDDepositorInsertSniper-Cas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationF539S, M763I, K890NPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa-V106W
Plasmid#193838PurposeExpress TadCBEa-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa V106W-SpCas9 D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBUN411
Plasmid#50581PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInserts3×FLAG-NLS-zCas9-NLS
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSExpressionMutationmaize codon optimizedPromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRM98 mKate2 Blast
Plasmid#174720PurposeExpresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.DepositorInsertsmKate2
BSD
UseLentiviralTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianMutationPromoterMND and MND (off mKate-P2A)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP11C-HA
Plasmid#209220PurposeMammalian expression of ATP11CDepositorInsertATP11C (ATP11C Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP9A-HA
Plasmid#209213PurposeMammalian expression of ATP9ADepositorInsertATP9A (ATP9A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEc
Plasmid#193834PurposeExpress TadCBEc (with SpCas9) in mammalian cellsDepositorInsertTadA-CDc-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GTB
Plasmid#26197DepositorInsertEnhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationTVA, rabies glycoprotein are mammalian codon opti…PromoterAvailable sinceNov. 11, 2010AvailabilityAcademic Institutions and Nonprofits only