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Plasmid#140735PurposeLentiviral vector to overexpress the autism-associated PTEN mutation D252G as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationD252GPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUG-PtenH93R
Plasmid#140733PurposeLentiviral vector to overexpress the autism-associated PTEN mutation H93R as a GFP-PTEN fusion proteinDepositorInsertGFP-PTEN (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationH93RPromoterhUbiCAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB0238-TEV-His12
Plasmid#137038Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66635.2
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-nsGFP
Plasmid#132975Purposegata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backboneDepositorInsertsnuclear localized sfGFP
gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2
Plasmid#111419Purposeexpress murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2+IA
Plasmid#111418Purposeexpress murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2+IA
Plasmid#111273Purposeexpress murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD2034
Plasmid#101377PurposeName: pBluescript-Δ611-843REV. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV.DepositorInsertBlue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
UseUnspecifiedTagsExpressionMutationBlue Heron Biotechnology Inc. bidirectional mutan…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF130
Plasmid#96971PurposeS. pombe lem2 knockout cassette with hygro resistance geneDepositorInsertlem2 (lem2 Fission Yeast)
UseTagsExpressionBacterialMutationwhole ORF deletion and replaced with hygro resist…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF169
Plasmid#96996Purposeish1 mCherry insertion cassette with G418 resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + G418 …PromoterN/AAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgGLDC_2
Plasmid#72887PurposeCRISPR-Cas9 induced gene disruptionDepositorInsertsgGLDC
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgGLDC_1
Plasmid#72886PurposeCRISPR-Cas9 induced gene disruptionDepositorInsertsgGLDC
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 1278-1291
Plasmid#59830PurposeAllows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 1278-1291Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mouse Nanog locus 3' probe
Plasmid#60037PurposeCloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysisDepositorInsertNanog (Nanog Mouse)
UseUnspecified; CloningTagsExpressionMutationPromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hoxd13-3'UTR
Plasmid#31525DepositorInsertHoxd13-3'UTR (Hoxd13 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
3691 pGEX4T-3 MLL AD mut.A
Plasmid#8863DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationILPSDIMDFVL --> ILPSDIMDAAAPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
3692 pGEX4T-3 MLL AD mut.B
Plasmid#8864DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationILPSDIMDFVL --> ILPSAAAAFVLPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
3693 pGEX4T-3 MLL AD mut.C
Plasmid#8865DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationILPSDIMDFVL --> ILPSNIMNFVLPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
3694 pGEX4T-3 MLL AD mut.D
Plasmid#8866DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationILPSDIMDFVL --> AAPSDIMDFVLPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
3696 pGEX4T-3 MLL AD I2844A
Plasmid#8868DepositorInsertMLL min A.D. (KMT2A Human)
UseTagsGSTExpressionBacterialMutationI2844A: ILPSDIMDFVL --> ALPSDIMDFVLPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only