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Plasmid#89410PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationaa 465-566PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-mCALEBlong.cyt (aa 522-594)
Plasmid#89409PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationaa 522-594PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-mCALEBcyt (aa 465-539)
Plasmid#89408PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationaa 465-539PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-chCALEBcyt (aa 522-551)
Plasmid#89407PurposeTwo-hybrid systemDepositorInsertCSPG5 (CSPG5 Chicken)
UseTagsGAL4 DBDExpressionYeastMutationaa 522-551PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-mCALEBsplice (aa 447-566)
Plasmid#89405PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
UseTagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Budding Yeast, Mouse)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F2-empty vector
Plasmid#125552PurposeN2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 alone without any attached prot…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-F1-empty vector
Plasmid#125551PurposeN2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 alone without any attached prot…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-E343A
Plasmid#171378PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationE343APromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSII424
Plasmid#35466DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSII423
Plasmid#35464DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-hphMX6
Plasmid#19342DepositorInserthphMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS40P
Plasmid#35481DepositorTypeEmpty backboneUseYeast integrating vectorTagsExpressionMutationPromoterAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSII426
Plasmid#35470DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-bleMX6
Plasmid#19344DepositorInsertbleMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-5FLAG-natMX6
Plasmid#19343DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only