pGBT9-mCALEBsplice (aa 447-566)
(Plasmid
#89405)
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PurposeTwo-hybrid system
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGBT9
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Backbone manufacturerClontech
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCSPG5
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Alt nameChondroitin Sulfate Proteoglycan 5
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Alt nameCALEB
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SpeciesM. musculus (mouse)
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Entrez GeneCspg5 (a.k.a. Caleb, Ngc)
- Promoter ADH1
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Tag
/ Fusion Protein
- GAL4 DBD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer Gal4-N-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBT9-mCALEBsplice (aa 447-566) was a gift from Fritz Rathjen (Addgene plasmid # 89405 ; http://n2t.net/addgene:89405 ; RRID:Addgene_89405)