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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Use

Tags

FLAG and NLS

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mAIM2-PYD-EGFP

Plasmid

#51610

Purpose

Express GFP-tagged PYD of mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of HIN domain

Promoter

MESV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28BdelN70

Plasmid

#51374

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28B (LIN28B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 70 amino acids deletion

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-DKAR-Nuc

Plasmid

#45499

Depositor

Alexandra Newton

Article

Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

Insert

DKAR-Nuc

Use

Tags

CFP, NLS, and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

hsp70

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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3158 pSFFV-neo Bad cDNA

Plasmid

#8753

Depositor

Stanley Korsmeyer

Article

Yang et al Cell 1995 Jan 27;80(2):285-91.

Insert

BAD (Bad Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pCMV5 KSR1 S392A

Plasmid

#25976

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/nV5-DEST-Apr1

Plasmid

#20135

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pTX1-SMN1-exon7-ESE1

Plasmid

#126041

Purpose

In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN1 exon7 ESE1

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S669A

Plasmid

#19282

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc S669A (Daxx Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
sox10:PDGFRA mutant

Plasmid

#98697

Purpose

I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Tags

Expression

Mutation

exon 8 and 9 deletion

Promoter

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL blast goosecoid

Plasmid

#14095

Depositor

Bob Weinberg

Article

Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

goosecoid (Gsc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-RFP-miR-223

Plasmid

#97148

Purpose

miR-223 expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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3XFlag-TMCC1

Plasmid

#121045

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Use

Tags

3x-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

Plasmid

#72240

Purpose

Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

GLT1 GAL1p 24xPP7 V3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJZ001

Plasmid

#120230

Purpose

Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

intB13 integrase casette

Use

Tags

Expression

Bacterial

Mutation

bla-g717a (amp resistance gene)

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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myc-hIFITM3-delta1-21

Plasmid

#104365

Purpose

Expresses human IFITM3-delta1-21 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095.

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

deleted amino acids 1-21

Promoter

CMV

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-deltaPYD-GST

Plasmid

#51534

Purpose

Express GST-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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3927-64

Plasmid

#176500

Purpose

pTRE-EGFP-T2A-TFEB(delta30; amino acids 31-476)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(human; delta30; amino acids 31-476)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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