Addgene

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pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FNIP2

Plasmid

#72295

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCAG/ATP11B-HA

Plasmid

#209219

Purpose

Mammalian expression of ATP11B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP11B (ATP11B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10D-HA

Plasmid

#209217

Purpose

Mammalian expression of ATP10D

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP10D (ATP10D Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Long P2A

Plasmid

#164109

Purpose

Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM-Mt-ER Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pbabe-hTERT+p53DD

Plasmid

#11128

Depositor

Christopher Counter

Article

Kendall et al Cancer Res. 2005 Nov 1. 65(21):9824-8.

Insert

hTERT, p53 dominant negative (TERT, Trp53 Human, Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

p53 dominant negative (aa1-14; 303-390)

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-M

Plasmid

#182284

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Short P2A

Plasmid

#164110

Purpose

Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM- Mt-ER Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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WWP-Luc (p21/WAF1 promoter)

Plasmid

#16451

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 promoter (CDKN1A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P199L

Plasmid

#177949

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P199L) (N )

Use

Tags

Expression

Mammalian

Mutation

P199L

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHSN401

Plasmid

#50588

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

3×FLAG-NLS-zCas9-NLS
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Expression

Mutation

maize codon optimized

Promoter

2×35Sp and AtU6-26p

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B2-HA

Plasmid

#209210

Purpose

Mammalian expression of ATP8B2

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B2 (ATP8B2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-L

Plasmid

#182283

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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AP-1 mu1-GFP

Plasmid

#173432

Purpose

Expression of AP-1 Mu1 subunit with a terminal GFP

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP1M1 (Ap1m1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

Plasmid

#160736

Purpose

Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B4-HA

Plasmid

#209212

Purpose

Mammalian expression of ATP8B4

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B4 (ATP8B4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only