-
Plasmid#183048PurposeExpresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.DepositorInsertAkaLuc
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV mKeima-LC3
Plasmid#189006PurposeFluorescent reporter for autophagyDepositorInsertmKeima, LC3 (Map1lc3b Rat)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 hPHGDH
Plasmid#154916PurposeDoxycyclin inducible expression of human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PABPC1_PABP
Plasmid#109931PurposeProtein expression and purification of PABPC1_PABPDepositorInsertPABPC1_PABP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-scFvGCN4sfGFPTET1CD
Plasmid#82561PurposeExpression vector for antibody-sfGFP-TET1CD.DepositorInsertscFvGCN4sfGFPTET1CD
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoCjCas9-BE4max-BlastR
Plasmid#194061PurposeMammalian expression of evoCjCas9 CBE4maxDepositorInsertevoCjCas9-BE4max
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
Plasmid#99248Purposesingle-vector system with EGFP reporter for use in CROPseq knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterEFSAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-H1 empty vector
Plasmid#25870Purpose3rd generation lentiviral vectorDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterH1Available sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
pINDUCER21-SOX4
Plasmid#51304PurposeExpression of SOX4 after doxycycline administrationDepositorInsertSRY (sex determining region Y)-box 4 (SOX4 Human)
UseLentiviralTagsn/aExpressionMutationfull length ORFPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.1
Plasmid#42631DepositorInsertHuman H3.1 (H3C1 Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLminP_Luc2P_RE60
Plasmid#90405PurposeSTAT5 - Stat5 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSIEAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Puro empty (w175-1)
Plasmid#17482Purpose3rd gen lentiviral empty control vector, CMV/TO promoter, PuroDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralTagsExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-mNeongreen
Plasmid#182389PurposeBacterial expression of Vesicle Nucleating peptide15-mNeongreen fusionDepositorInsertVNp15-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
V406 4HA-CKIe(K38R)
Plasmid#13725DepositorInsertcasein kinase 1 epsilon (CSNK1E Human)
UseTags4 HAExpressionMammalianMutationkinase-inactive due to K38R mutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
mGeos-M-Homer1-C-18
Plasmid#57528PurposeLocalization: Membrane Adaptor, Excitation: 503 / 569, Emission: 514 / 581DepositorInsertHomer1 (Homer1 Rat)
UseTagsmGeos-MExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVpuro-Mito-DsRed1
Plasmid#87379PurposeRetroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).DepositorInsertTXN2 mitochondrial transit peptide (TXN2 Human)
UseRetroviralTagsDsRed1ExpressionMammalianMutationFirst 60 aa onlyPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-eGFP-dSaCas9-blast
Plasmid#191653PurposeLentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.DepositorInserteGFP-T2A-dSaCas9
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
retro-gRNA-eGFP
Plasmid#116926PurposeRetrovirus expressing eGFP with BBSI cloning sites for sgRNADepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD276-Fc(DAPA)-AviTag-6xHis
Plasmid#156861PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD276 (CD276 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-EGFP
Plasmid#80945Purposehuman AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoterDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-5xTAPE-1-BlastR
Plasmid#175808PurposeEncodes 5xTAPE-1 construct that can be integrated using piggyBAC systemDepositorInsert5xTAPE-1 construct
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 RARE-RFP
Plasmid#183054PurposeFluorescent reporter for Retinoic Acid activityDepositorInsertRFP
UseTagsExpressionMutationPromoterRARE, SV40Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Stat3-C Flag pRc/CMV
Plasmid#8722DepositorInsertStat3-C (Stat3 Mouse)
UseTagsFlagExpressionMammalianMutationA662C and N664C. Residues changed to Cys renders…PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
T-2A-EGFP-PGK-Puro
Plasmid#83344PurposeDonor vector to knocin EGFP into human Brachyury(T) locusDepositorInsertHuman Brachyury knockin homology arms (TBXT Human)
UseTags2A-EGFPExpressionMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
Plasmid#84239PurposeExpresses ABA-inducible VPR-Sp dCas9DepositorInsertsABI-tagBFP-Sp dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only