pMSCVpuro-Mito-DsRed1
(Plasmid #87379)

• Purpose: Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
• Depositing Lab: Björn Stork
• Publication: Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. doi: 10.1038/s41419-018-0312-8.

Backbone

• Vector backbone: pMSCVpuro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6264
• Total vector size (bp): 7260
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TXN2 mitochondrial transit peptide
• Alt name: Thioredoxin
• Species: H. sapiens (human)
• Insert Size (bp): 180
• Mutation: First 60 aa only
• GenBank ID: NM_012473.3
• Entrez Gene: TXN2 (a.k.a. COXPD29, MT-TRX, MTRX, TRX2, TXN)
• Promoter: Retroviral 5' LTR promoter
• Tag / Fusion Protein:
  • DsRed1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pMSCV-F
• 3′ sequencing primer: pMSCV-R

Resource Information

• Supplemental Documents:
  • PB209.gb
• A portion of this plasmid was derived from a plasmid made by: mito-DsRed1 was obtained from Prof. Du Feng (Guangzhou Medical University, China)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry