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Plasmid#113620PurposesgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reportersDepositorInsert7a sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRM209 LTP (Luciferase tdTomato Puro)
Plasmid#174723PurposeExpresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
pac
UseLentiviral and LuciferaseTagsE2A linker and T2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-mCherry-FRB
Plasmid#172443PurposeExpression of plasma membrane protein Stargazin tagged with mCherry-FRBDepositorInsertCacng2 (Cacng2 Mouse)
UseTagsmCherry-HA-FRBExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X
Plasmid#192148PurposeT cell CRISPR AAV vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-TDP-43 sgRNA1
Plasmid#133762PurposeExpresses Cas9 and human TDP-43 sgRNADepositorInsertTDP-43 (TARDBP Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycYBX1
Plasmid#19878DepositorInsertY box binding protein 1 (YBX1 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-NeoR-CAG-EGFPnls-WPRE
Plasmid#191692PurposeFor knocking pCAG-EGFPnls into AAVS1 locusDepositorInsertAAVS1 homology arms and pCAG-EGFPnls
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR Lck-TagBFP
Plasmid#171034PurposeTagBFP-tagged Lck kinaseDepositorInsertLck
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pHR-tdPP7-3xmCherry
Plasmid#74926PurposeExpresses tdPP7-3xmCherryDepositorInsertPP7-3xmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mito-PAGFP
Plasmid#23348PurposeExpresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.DepositorInsertPAGFP
UseTagsmitoExpressionMammalianMutationPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUK21
Plasmid#49788PurposeE. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterlacZAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
UseTagsExpressionYeastMutationPromoterAlcohol OxidaseAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP TFEB-sfGFP
Plasmid#135402PurposeExpresses TFEB-sfGFPDepositorInsertTFEB (TFEB Human)
UseRetroviralTagssfGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.pRSET
Plasmid#33372Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertMBP based Maltose Biosensor
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-scFv-T
Plasmid#67843PurposepET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.DepositorInsertantibody 1D8 light kappa chain
UseTagsHis-tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPble
Plasmid#63561PurposeBleomycin drug selection markerDepositorInsertBleomycin drug selection marker
UseLeishmania expressionTagsExpressionMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9TagsExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-NpHR3.3-EYFP
Plasmid#137152PurposeIntersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-NpHR3.3-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only