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Plasmid#71754Purpose+36GFP-LPETG for expression in E.ColiDepositorInsert(GGS)9_(pos36GFP)_6XHis_LPETG
UseTags6XHis and Sortase (LPETG)ExpressionBacterialMutationPromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPet-His
Plasmid#14031DepositorInsertYPet
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN
Plasmid#221348PurposeExpress ChmN in E. coliDepositorInsertchmN
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
UseTagspolyhistidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24b-Amg2
Plasmid#199434Purposeexpresses Amg2 in bacterial cellsDepositorInsertAmg2
UseTagshis tagExpressionBacterialMutationL159K mutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO3
Plasmid#53143PurposeE.coli expression of untagged mature human SUMO3 (amino acids 1-93)DepositorInsertSUMO3 (SUMO2 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-93)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4YPet-MAMM
Plasmid#14032DepositorInsertYPet
UseTagsExpressionMammalianMutationMammalian optimized YPetPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDSalI
Plasmid#38251DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH4
Plasmid#71713PurposeMembrane scaffold protein (MSP), helix 4 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Rhodoplanes-bGSDM
Plasmid#212129PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRhodoplanes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM
Plasmid#212124PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBacteroidetes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Runella-bGSDM
Plasmid#212123PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRunella bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bradyrhizobium-bGSDM
Plasmid#212126PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBradyrhizobium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-dAsCas12f
Plasmid#204641PurposeExpression of an inactive AsCas12f in E. coliDepositorInsertinactive AsCas12f
UseTags6xHisExpressionBacterialMutationD225APromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_sfCherry(1-10)_32aalinker_sfCherry(11)
Plasmid#83030PurposeBacterial expression vector for split sfCherry screeningDepositorInsertsfCherry(1-10)_32aalinker_sfCherry(11)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcYbbN pET15b
Plasmid#60690PurposeRecombinant protein expression of YbbNDepositorInsertYbbN
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only