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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEP4
- Backbone size w/o insert (bp) 10410
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYPet
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MutationMammalian optimized YPet
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer See map (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The assembled sequence for this plasmid is inaccurate. The plasmid is actually 2.3kb smaller than the sequence suggests.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP4YPet-MAMM was a gift from Patrick Daugherty (Addgene plasmid # 14032 ; http://n2t.net/addgene:14032 ; RRID:Addgene_14032) -
For your References section:
Evolutionary optimization of fluorescent proteins for intracellular FRET. Nguyen AW, Daugherty PS. Nat Biotechnol. 2005 Mar . 23(3):355-60. 10.1038/nbt1066 PubMed 15696158