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Showing: 601 - 620 of 892 results

  6. pSSA-DsRed/eGFP (pXK-442)

    Plasmid
    #208827
    Purpose
    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208827-plasmid-map-sequence-id-417251

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  7. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

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  8. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  9. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid
    #179526
    Purpose
    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 KES(1107-1109)GG
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    replaced KES (residues 1107-1109) with GG
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179526-plasmid-map-sequence-id-351748

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  10. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

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  12. PV259

    Plasmid
    #132340
    Purpose
    CasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    CasD-AT-CBF1 (CBF1 CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132340-plasmid-map-sequence-id-258453

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  13. PV503

    Plasmid
    #132342
    Purpose
    CasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    CasE-AT-CBF1 (CBF1 CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132342-plasmid-map-sequence-id-258473

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  14. PV504

    Plasmid
    #132343
    Purpose
    CasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays
    Depositor
    Joshua Young
    Article
    Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.
    Insert
    CasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
    Use
    Tags
    AT-CBF1
    Expression
    Plant
    Mutation
    Codon optimize for expression and stability in Ze…
    Promoter
    Available since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    132343-plasmid-map-sequence-id-258480

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  15. pJOG252

    Plasmid
    #80584
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80584-plasmid-map-sequence-id-151870

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  16. pJOG253

    Plasmid
    #80585
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80585-plasmid-map-sequence-id-151872

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Showing: 601 - 620 of 892 results