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Plasmid#79382PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertChimera 4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…PromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
UseTagsCeruleanExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin
Plasmid#223965PurposeDual fluorescent reporter for histone H2B and for Cdt1DepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-IGKleader-hIgG1_FC-Myc-PDGFRb-IRES2-EGFP (MCS reporter)
Plasmid#222998PurposeMCS reporter constitutively expressing EGFP and conditionally expressing a synthetic cell surface protein upon prime editing. For lentivirus packaging.DepositorInsertIGKleader-hIgG1_FC-Myc-PDGFRb-IRES2-EGFP
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204817PurposeBacterial expresison of Enterovirus D68 3C proteaseDepositorInsertEV D68 3C protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX
Plasmid#214278PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationCdc42-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX
Plasmid#214279PurposeExpress split-Cdc42 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationCdc42-T17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX
Plasmid#214280PurposeExpress split-Rac1 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationRac1-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX
Plasmid#214281PurposeExpress split-Rac1 fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRac1-T17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX
Plasmid#214282PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationRhoA-Q63LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX
Plasmid#214283PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRhoA-T19NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX
Plasmid#214284PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationKRas-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX
Plasmid#214285PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationKRas-S17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX
Plasmid#214286PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationKRas-Q61LPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX
Plasmid#214287PurposeExpress split-KRas fragments that are fused with CIDs and FPsDepositorInsertsUseTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationKRas-S17NPromoterCMVAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only