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Plasmid#189559PurposeProtein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-459PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CLPIT Cry2PHR-mCh-RhoA
Plasmid#42959DepositorInsertCry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
UseRetroviralTagsExpressionMammalianMutationPromoterTet-Off promoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IL6ST-COMP5AP-AviTag-9xHis
Plasmid#157485PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertIL6ST (IL6ST Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EK0501 SFFV-mCherry-SGc(MYC.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191185PurposeExpression of MYC sensor in mammalian cellsDepositorInsertmCherry:MYC.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-TRIM28 ts3
Plasmid#115864PurposeTRIM28 knockdownDepositorInsertTRIM28 shRNA (TRIM28 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mTagBFP2-A_UTR
Plasmid#68479PurposeLentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mTagBFP2-A_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "A" added to 3…PromoterCMV and pGKAvailable sinceNov. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCerulean
Plasmid#99268PurposeContains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcriptionDepositorInsertmCerulean-3XFLAG
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGF012
Plasmid#48849PurposeProvides a sulfadiazine resistance cassette as GreenGate module.DepositorInsertpMAS::SulfadiazineR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition sites in SulfR removed …PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME18S-F-R-Olfr160
Plasmid#149366PurposeExpresses Olfr160 in mammalian cells.DepositorInsertOlfr160 (Olfr160 Mouse)
UseTagsFLAG and rhodopsinExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV1825
Plasmid#37838DepositorInsertPnhx-2DsRed::KDEL
UseTagsKDELExpressionWormMutationPromoternhx-2Available sinceDec. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJH1579
Plasmid#178904PurposePglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60DepositorInsertYC3.60
UseTagsExpressionWormMutationSee Depositor Comments BelowPromoterPglr-1Available sinceJan. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAPM-D4 miR30-TRIM28 ts1
Plasmid#115862PurposeTRIM28 knockdownDepositorInsertTRIM28 shRNA (TRIM28 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ2076.2_cL
Plasmid#140668PurposecL control for toggle switch experimentDepositorInsertcL control for toggle switch experiment
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
Plasmid#108833PurposeExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDsDepositorInsertFlp recombinase fragment aa28 to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLS and GAIExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)
Plasmid#225752PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
OgeuIscB ωRNA-v13
Plasmid#219634Purposehuman U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloningDepositorInsertOgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB
Plasmid#219636PurposeCMV-SV40 NLS-enOgeuIscB-nucleoplasmin NLSDepositorInsertCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX459v3
Plasmid#178799PurposeSpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP
Plasmid#219828PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIQ-EGFP
Plasmid#216239PurposeAll-in One IQ transgene switch for EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR2-DuET
Plasmid#213198PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR2 (CCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG9A HRD
Plasmid#203222PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cellsDepositorInsertHaloTag flanked by human ATG9 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GABBR1-DuET
Plasmid#213247PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGABBR1 (GABBR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EDNRB-DuET
Plasmid#213233PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertEDNRB (EDNRB Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
F2R-DuET
Plasmid#213234PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertF2R (F2R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR2C-DuET
Plasmid#213323PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertHTR2C (HTR2C Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPRC5D-DuET
Plasmid#213298PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPRC5D (GPRC5D Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EDNRA-DuET
Plasmid#213232PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertEDNRA (EDNRA Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR6-DuET
Plasmid#213223PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR6 (CXCR6 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR8-DuET
Plasmid#213204PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR8 (CCR8 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR5-DuET
Plasmid#213201PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR5 (CCR5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
UseTagsbdSUMOExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11
Plasmid#184277PurposeExpresses nanobody H11 in E. coliDepositorInsertNanobody H11
UseTagsHis6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6
Plasmid#198408PurposeExpresses FLAG-tagged human IntS6DepositorInsertDDX26 (INTS6 Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-KIF20A zeo
Plasmid#192322PurposeLentiviral expression vector for an inducible 3xFLAG-KIF20ADepositorInsertKIF20A (KIF20A Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-Fluoro-Phe D6
Plasmid#197577PurposeExpression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cellsDepositorInsertsMb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)
UseTagsNuclear export sequence + FLAGExpressionMammalianMutationN311A, C313M, V366G, W382TPromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-Fluoro-Phe B5
Plasmid#197576PurposeExpression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cellsDepositorInsertsMb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)
UseTagsNuclear export sequence + FLAGExpressionMammalianMutationN311G, C313APromoterCMV and U6/H1Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_CD8alpha variant 4
Plasmid#188364PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant4-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant4-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYap1-1
Plasmid#193669PurposeTet inducible knockdown of Yap1DepositorInsertYap1 (Yap1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shYAP1-1
Plasmid#193692PurposeConstitutive lentiviral expression of YAP1 shRNADepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA13a-RFP
Plasmid#126086PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23102Available sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MORC2 ts2
Plasmid#115887PurposeMORC2 knockdownDepositorInsertMORC2 shRNA (MORC2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cyto-roGFP
Plasmid#49435PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cellsDepositorInsertroGFP2
UseAdenoviralTagsExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterT7 promoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only