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Showing: 6161 - 6180 of 15727 results

  12. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al bioRxiv 2024.07.19.604224
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  13. pCMV-eGFP-mPlum-HA

    Plasmid
    #200009
    Purpose
    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.
    Depositor
    Thomas Noll
    Article
    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:
    Insert
    eGFP, mPlum
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200009-plasmid-map-sequence-id-398431

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  14. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

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Showing: 6161 - 6180 of 15727 results