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Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
UseTagsExpressionBacterialMutationPromoterPatoLAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoK-LOG241
Plasmid#72848PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX302 PCSK5 (T288P)-V5 puro
Plasmid#98709PurposeLentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tagDepositorInsertPCSK5 (PCSK5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationChanged Threonine 288 to ProlinePromoterAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a V52A
Plasmid#90050PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT/V52A
UseTagsExpressionBacterialMutationV52APromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a Q55A
Plasmid#90052PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA/Q55A
UseTagsExpressionBacterialMutationQ55APromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhC-pHG165a Q55I
Plasmid#90053PurposeConstitutive expression of a chimeric LacI:CytR transcription repressorDepositorInsertLLhC/Q55I
UseTagsExpressionBacterialMutationQ55IPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Cav2.2 DomI and DomII
Plasmid#89891PurposeMammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2DepositorInsertCacna1b (CACNA1B )
UseTagsExpressionMammalianMutationStop codon after amino acid 1154 (this is a trunc…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-a4-delta tail
Plasmid#80018PurposeMammalian expression of human integrin alpha4 with no tailDepositorInsertintegrin alpha4 (ITGA4 Human)
UseTagsExpressionMammalianMutationno cytoplasmic tail (amino acids 1 to 1007)PromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H09
Plasmid#71003PurposeGateway entry cloneDepositorInsertSNF4Agamma (SNF4Agamma Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4F04
Plasmid#71030PurposeGateway entry cloneDepositorInsertSin3A (Sin3A Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2B09
Plasmid#70918PurposeGateway entry cloneDepositorInsertgro (gro Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2C08
Plasmid#70911PurposeGateway entry cloneDepositorInsertTaf5 (Taf5 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3C03
Plasmid#70959PurposeGateway entry cloneDepositorInsertTaf4 (Taf4 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2C05
Plasmid#70891PurposeGateway entry cloneDepositorInsertOrc2 (Orc2 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorUseTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL11062
Plasmid#68256PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160S,HisTagDel)
Plasmid#66880PurposeExpresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160S)
UseTagsnoneExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-B
Plasmid#64514PurposeAS-30D hepatoma hexokinase II 3.0 kbp promoter-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase IIAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq20-T38S38-0X
Plasmid#49522PurposeKanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertconstitutive TetR38
UseTagsExpressionBacterialMutationPromoterPtb38Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only