Addgene

Showing: 6121 - 6140 of 11722 results

Results per page:

Sort results by:

pDEST myc MOV10 D645N

Plasmid

#51720

Purpose

plasmid expresses myc-tagged MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

D645N

Promoter

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-CUTC

Plasmid

#34801

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

cutC copper transporter homolog (E. coli) (CUTC Human)

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mTFP1-Tubulin-6

Plasmid

#55512

Purpose

Localization: Microtubules, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Tubulin (TUBA1B Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter hsa-mir-138-2

Plasmid

#46677

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-138-2 (MIR138-2 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Plxnb2-ISH-2kb

Plasmid

#68036

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Mrna in situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-COASY

Plasmid

#34816

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

Coenzyme A synthase (COASY Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Sema4c-ISH-2kb

Plasmid

#68031

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4C (Sema4c Mouse)

Use

Mrna in situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe_puro_DEST_Flag_SLC7A14

Plasmid

#45261

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SLC7A14 (SLC7A14 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-Nup35-mut

Plasmid

#87341

Purpose

To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP35 (NUP35 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Tags

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

Plasmid

#117910

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44A on eDHFR

Promoter

T7

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

Plasmid

#117909

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R98A on eDHFR

Promoter

T7

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 S103L (#624)

Plasmid

#99942

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S103L

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL N-Myc-hPOT1_deltaOB

Plasmid

#166408

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

ΔOB

Promoter

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL N-Myc-hPOT1_IRES_hygro

Plasmid

#166406

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-SIRT3

Plasmid

#66167

Purpose

Expresses SIRT3 via retroviral method

Depositor

Ronald Kahn

Article

Jing et al Diabetes. 2013 Oct;62(10):3404-17. doi:

Insert

SIRT3 (Sirt3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L235P

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#67608

Purpose

bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

catalytic mutant LDIG(182-185)AAAA

Promoter

tac

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge