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GST-PRMT9 L182A/D183A/I184A/G185A
(Plasmid #67608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Backbone manufacturer
    GE healthcare
  • Backbone size w/o insert (bp) 4984
  • Total vector size (bp) 7518
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PRMT9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2538
  • Mutation
    catalytic mutant LDIG(182-185)AAAA
  • GenBank ID
    NM_138364.3
  • Entrez Gene
    PRMT9 (a.k.a. PRMT10)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5GEX
  • 3′ sequencing primer 3GEX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-PRMT9 L182A/D183A/I184A/G185A was a gift from Steven Clarke (Addgene plasmid # 67608 ; http://n2t.net/addgene:67608 ; RRID:Addgene_67608)

  • For your References section:

    Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and its Substrate RNA Splicing Factor SF3B2. Hadjikyriacou A, Yang Y, Espejo A, Bedford MT, Clarke SG. J Biol Chem. 2015 May 15. pii: jbc.M115.659433. 10.1074/jbc.M115.659433 PubMed 25979344
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