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Addgene
Showing: 6101 - 6120 of 12974 results
  1. pYES2-gRNA-hyg-MCS

    Plasmid
    #107734
    Purpose
    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.
    Depositor
    Insert
    X-3 site gRNA expression cassettes
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SNR52p
    Availability
    Academic Institutions and Nonprofits only
  2. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  3. pX330.orup

    Plasmid
    #110404
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  4. pX330.puro sgRNA KGA Stop1

    Plasmid
    #110405
    Purpose
    CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  5. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid
    #110624
    Purpose
    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
    Depositor
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    LP1B and hU6
    Availability
    Academic Institutions and Nonprofits only
  7. U6_sgRNA_CAG_hSt1Cas9_LMD9

    Plasmid
    #110626
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
    Depositor
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Availability
    Academic Institutions and Nonprofits only
  8. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Availability
    Academic Institutions and Nonprofits only
  9. pCSDC2.1.0-gDNA

    Plasmid
    #113781
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2
    Depositor
    Insert
    CSDC2 (CSDC2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pHOXB13.1.0-gDNA

    Plasmid
    #113782
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB13
    Depositor
    Insert
    HOXB13 (HOXB13 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 6101 - 6120 of 12974 results